Segmentation of cell nuclei in fluorescence microscopy images: An integrated framework using level set segmentation and touching-cell splitting

作者：

Highlights：

• Cell segmentation using level sets based segmentation algorithm and a touching-cell splitting method.

• Experimental evaluation using fluorescence microscopy images from different datasets with different cell types.

• The proposed approach can cope with touching cell nuclei and images with intensity inhomogeneity.

摘要

•Cell segmentation using level sets based segmentation algorithm and a touching-cell splitting method.•Experimental evaluation using fluorescence microscopy images from different datasets with different cell types.•The proposed approach can cope with touching cell nuclei and images with intensity inhomogeneity.

论文关键词：Fluorescence microscopy images,Level set segmentation,Touching-cell splitting

论文评审过程：Received 21 July 2015, Revised 30 January 2016, Accepted 28 March 2016, Available online 6 April 2016, Version of Record 26 May 2016.

论文官网地址：https://doi.org/10.1016/j.patcog.2016.03.030