A method of boundary determination in digital images of urothelial cells

摘要

Digital images acquired by high resolution scanning of urothelial cells are segmented into nuclear and cytoplasmic regions. A method which is a hybrid of a gradient tracking method and a thresholding method is used, and criteria are presented to automatically detect failures of the algorithm. Tests on a sample of benign and malignant urothelial cells show a significant improvement in both accuracy and reliability as compared with a single segmentation method.