Application of Gröbner basis techniques to enzyme kinetics

摘要

It is possible to write down the equations governing a one-stage enzyme-catalysed reaction (according to Michaelis–Menten kinetics) quite easily, and deduce information about the steady-state flux in such a system. The situation is somewhat more complicated if several such reactions form a linear chain. We have applied Gröbner basis techniques to solve such systems and hence demonstrate the use of computer algebra as a powerful technique in enzyme kinetic analysis.