3D representation of biostructures imaged with an optical microscope: Part A: Digital optical sectioning

摘要

A method for acquiring and processing 3D information from biological specimens imaged with an optical microscope is presented. It is physically based on optical sectioning microscopy, and utilizes a set of digital filters implemented for deblurring optical section images. Rather than the provision of an exact solution for the problem, we have found of more practical use a new approximate method that significantly improves the situation, within reasonable cost parameters.